Journal: bioRxiv

Article Title: Restoring Shugoshin 1 reduces chromosome errors in human eggs

doi: 10.64898/2026.01.08.698387

Figure Lengend Snippet: (A) Representative immunofluorescence images showing the localization nascent transcripts (EU, green) inside the nucleus (blue) of early GV oocytes. Images show EU levels in negative control (no EU), control (with EU), upon treatment with α-amanitin, and triptolide. Scale bar: 10 µm. (B) Frequency of PSSC with α-amanitin measured by scoring the percentage of eggs with PSSC, similar to levels observed with triptolide . Plots show number of eggs analyzed on top. Statistical significance is measured by Fisher’s exact test, ns = not significant. (C) Representative time-lapse images showing the localization of MajSat RNA in GV and MI oocytes and MII eggs upon microinjection of GV oocytes with UTP-X-Cy3 labeled MajSat (top panel) and control (bottom panel) RNA. MajSat RNA localizes to the pericentromeres during metaphase I and metaphase II stages along with foci present in the cytoplasm. Cy3-labeled control RNA localizes only in the cytoplasm and not on chromosomes. MajSat and control RNA (green), chromosomes (H2B-SNAP, blue) are shown. Scale bars: 10 µm. (D) Co-localization of MajSat RNA and SGO1 observed on metaphase I chromosome spreads upon performing RNA FISH with immunofluorescence. Scale bar: 10 µm.

Article Snippet: The HighYield T7 Cy3 RNA Labelling Kit (Jena Bioscience, #RNT-101-CY3) was used to label amplified sequences with Cy3-labelled UTP (UTP-X-Cy3, 35%), followed by DNA removal using TurboTM DNAse (ThermoFisher).

Techniques: Immunofluorescence, Negative Control, Control, Microinjection, Labeling

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

doi: 10.1007/s00018-025-06055-3

Figure Lengend Snippet: Effect of inflammation on spermatogonia in LPS-induced mouse testes. ( A ) Testes from LPS-induced mice (Orchitis) and the Sham group. n = 6 mice per group ( B ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected using qPCR. n = 6 mice per group ( C ) Expression of SP1 at the protein level as detected by Western blot. n = 6 mice per group ( D ) H&E staining was used to analyze the histopathology of the testes. n = 3 mice per group ( E ) Apoptosis in testes was analyzed using TUNEL staining. n = 3 mice per group (F-I) Expression levels of Oct4, C-kit, IL-17 A and ZO-1 as detected by IHC assay. n = 3 mice per group. Statistical comparisons between the two groups were performed using an unpaired two-tailed Student’s t-test. *** P < 0.001

Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

Techniques: Expressing, Western Blot, Staining, Histopathology, TUNEL Assay, Two Tailed Test

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

doi: 10.1007/s00018-025-06055-3

Figure Lengend Snippet: Effects of LPS-induced inflammation on spermatogonia in vitro. (A) Cell proliferation was detected by EdU staining. (B-C) Apoptosis was detected using flow cytometry and the TUNEL assay. (D) SP1 expression at the protein level was detected using a Western blot. (E) Expression levels of SNHG1 and SP1 at the transcriptional level were detected using qPCR. (F–H) IF analysis of SP1 , C-kit, and Oct4 expression. All experiments were performed in at least triplicate. Statistical comparisons between the two groups were performed using an unpaired two-tailed Student’s t-test. ** P < 0.01, *** P < 0.001

Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

Techniques: In Vitro, Staining, Flow Cytometry, TUNEL Assay, Expressing, Western Blot, Two Tailed Test

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

doi: 10.1007/s00018-025-06055-3

Figure Lengend Snippet: SP1 knockdown reduces the LPS-induced spermatogonia dysfunction. ( A ) Cell proliferation was detected using EdU staining. ( B - C ) Cell apoptosis was detected using flow cytometry and the TUNEL assay. ( D ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected using qPCR. ( E ) SP1 expression at the protein level was detected by Western blot. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

Techniques: Knockdown, Staining, Flow Cytometry, TUNEL Assay, Expressing, Western Blot

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

doi: 10.1007/s00018-025-06055-3

Figure Lengend Snippet: SNHG1 knockdown reduces LPS-induced spermatogonial dysfunction. ( A ) Cell proliferation was detected by EdU staining. ( B - C ) Cell apoptosis was detected by flow cytometry and TUNEL assay. ( D ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected by qPCR. ( E ) SP1 expression at the protein level was detected by Western blot. ( F – H ) IF analysis of SP1 , C-kit, and Oct4 expression. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

Techniques: Knockdown, Staining, Flow Cytometry, TUNEL Assay, Expressing, Western Blot

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

doi: 10.1007/s00018-025-06055-3

Figure Lengend Snippet: IL-17A signaling pathway blockade rescued the SNHG1 -induced cell dysfunction. ( A ) Cell proliferation was detected using EdU staining. ( B - C ) Cell apoptosis detected by flow cytometry and TUNEL assay. ( D ) Expression levels of SNHG1 and SP1 at the transcriptional level were detected by qPCR. ( E ) SP1 expression at the protein level was detected using a Western blot. ( F – H ) IF analysis of SP1 , C-kit, and Oct4 expression. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, ** P < 0.01, *** P < 0.001

Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

Techniques: Staining, Flow Cytometry, TUNEL Assay, Expressing, Western Blot

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Inflammation-induced LncRNA SNHG1 orchestrates spermatogonium development in non-obstructive azoospermia via IL-17 A signaling pathway

doi: 10.1007/s00018-025-06055-3

Figure Lengend Snippet: Re-expressing SNHGI in SP1-knock-down cells protect cells from inflammatory damage. ( A - C ) Expression levels of SP1 , SNHG1 , and IL-17 A were detected by QPCR assay. ( D ) The WB method was employed to assess the protein expression levels of SP1 and IL-17 A . ( E ) EdU staining was utilized to evaluate cell proliferation. ( F ) Flow cytometry was applied to detect cell apoptosis. All experiments were performed in at least triplicate. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. ns: not significant, ** P < 0.01, *** P < 0.001

Article Snippet: For cell lines, the one-step TUNEL apoptosis assay kit with Cy3 (Beyotime, China) was used according to the manufacturer’s instructions.

Techniques: Expressing, Knockdown, Staining, Flow Cytometry